Limulus Amebocyte Lysate

Limulus Amebocyte Lysate (LAL)

Overview:

The activation of LAL by glucan in a sample can be prevented by adding a large amount of carboxymethylated curdlan (CMC) to LAL.
Pammvi Exports is the authorised suppliers, agents, dealers and wholesalers for Wako USA manufactured Limulus Amebocyte Lysate Reagent (Lal Reagent). This is USFDA licensed facility.

PYROSTAR™ ES-F Reagent: PYROSTAR™ ES-F is a lyophilized reagent containing Limulus amebocyte lysate, buffers, carboxy-methyl curdlan, monovalent and divalent cations.

Preparation: Tap the vial on a level surface to ensure that all powder is at the bottom of the vial. Gently remove the stopper and add 2 mL or 5.2 mL of LAL Reagent Water (LRW) to the vial. Replace the stopper and swirl gently to dissolve the contents without making contact to the stopper.

Storage: Store at 2 to 10oC. Refrigerate reconstituted reagent at 2-8°C, maximum 4 hrs or at -15±5oC for a maximum of 14 days. Reconstituted reagent can only be frozen and thawed one time.

However, the specificity of LAL is not absolute. It has been reported that LAL reacts not only with endotoxin by also with β-1,3-glucan. Although the cascade system activated by β-1,3-glucan has been shown to be different than the one activated by endotoxin, the end result, gel-clot formation is indistinguishable.

The activation of LAL by glucan in a sample can be prevented by adding a large amount of carboxymethylated curdlan (CMC) to LAL. The presence of large amounts of glucan does not interfere with the quantitation of endotoxin. Wako first made use of these findings by developing an ES-buffer, which contains high concentrations of CMC. When the ES-buffer is used to reconstitute LAL, the LAL reagent becomes endotoxin-specific. PYROSTAR™ ES-F is a new preparation of LAL in which CM-curdlan is colyophilized with LAL. Reconstituting PYROSTAR™ ES-F multi test vials with LAL Reagent Water results in an endotoxin-specific LAL reagent.

WPEK4-20015 - 0.015EU/ML
WPEK4-20003 - 0.03EU/ML
WPEK4-20006 - 0.06EU/ML
WPEK4-20125 - 0.125 EU/ML

WPEK4-50015 - 0.015EU/ML
WPEK4-50003 - 0.03EU/ML
WPEK4-50006 - 0.06EU/ML
WPEK4-50125 - 0.125 EU/ML