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          • Gluematic 3002
          • Gluematic 5000
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        • DOA & Troponin-I Reader
        • Alcohol Breath Analyser
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        • Occuloplasty
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          • Hearing Aids For Children
          • Modern Hearing Aids
          • Non Programmable Hearing Aids
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          • Download Hearing fitting system
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Melamine Elisa Test Kit

Home » Products » Melamine Elisa Test Kit

Melamine Elisa Test Kit

Overview:

Melamine is described as “Harmful if swallowed, inhaled or absorbed through the skin. Chronic exposure may cause cancer or reproductive damage. Eye, skin and respiratory irritant.” However, the short-term lethal dose is on a par with common table salt with an LD50 of more than 3 grams per kilogram of bodyweight.

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PROPERTIES

Melamine is described as “Harmful if swallowed, inhaled or absorbed through the skin. Chronic exposure may cause cancer or reproductive damage. Eye, skin and respiratory irritant.” However, the short-term lethal dose is on a par with common table salt with an LD50 of more than 3 grams per kilogram of bodyweight.

PRODUCT DESCRIPTION

Product Name: Melamine (MEL) ELISA Kit

Specification: 96T

Storage Condition: 2-8°C

Restriction: For research use only!

TECHNICAL SPECIFICATIONS

Component 96 wells
Antigen coated plate 12 test strips of 8 wells each
6 vials of standard 1ml each
High concentration standard 1ppm 1ml

HRP-conjugate

6ml
Antibody Solution 6ml
Substrate A 6ml
Substrate B 6ml
Stop Solution 6ml
Concentrated Sample redissolving solution 16X 12ml
Concentrated Wash Buffer (10×) 40ml X2
IFU 1
Testing Report 1

Sensitivity, accuracy and precision

Sensitivity:            1ppb

Limit of detection

Milk powder……………………………………………………………………………………50ppb

Yoghourt….……………………………………………………………………………………10ppb

Fresh Milk………………………………………………………………………………………50ppb

Feed….…………………………………………………………………………………………50ppb

Egg….………………………………………………………………………………………….10ppb

Percent Recovery, %

Milk powder…………………………………………………………………………………100±20%

Yoghourt….…………………………………………………………………………………100±20%

Fresh Milk……………………………………………………………………………………100±20%

Feed….………………………………………………………………………………………100±20%

Egg….………………………………………………………………………………………..100±20%

Precision (CV %):

Intra-lab assay: CV%≤10%

Melamine ELISA Kitis an indirect competitive enzyme-labeled immunoassay. The tetracycline antigen is precoated on the wells. The precoated antigen compete the tetracycline antibody (antibody solution) with tetracycline in the sample, anti- tetracycline antibody binds to the tetracycline-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the tetracycline concentration of the samples is derived.

Scope of Application

Qualitative or quantitative analysis the residues of Melaminein milk, milk powder, dairy, egg and feed, etc.

Cross-reactivity

Melamine…………………………………………………100%

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