PROPERTIES
PRODUCT DESCRIPTION
Product Name: Streptomycin(STR) ELISA Kit
Specification: 96T
Storage Condition: 2-8°C
Restriction: For resaerch use only!
Summary
Streptomycin is an antibiotic (antimycobacterial) drug, the first of a class of drugs called aminoglycosides to be discovered, and it was the first effective treatment for tuberculosis. It is derived from the actinobacterium Streptomyces griseus. Streptomycin is a bactericidal antibiotic.[2] Adverse effects of this medicine are ototoxicity, nephrotoxicity, fetal auditory toxicity, and neuromuscular paralysis.
Use Principles
Streptomycin ELISA Kitis an indirect competitive enzyme-labeled immunoassay. The tetracycline antigen is precoated on the wells. The precoated antigen compete the tetracycline antibody (antibody solution) with tetracycline in the sample, anti- tetracycline antibody binds to the tetracycline-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the tetracycline concentration of the samples is derived
TECHNICAL SPECIFICATIONS
Cross-reactivity
Streptomycin…………………………………………………100%
Dihydrostreptomycin Sulphate…………………………….100%
Kanamycin……………………………………………………<0.01%
Gentamycin………………………………………………..…<0.01%
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 6 vials of standard | 1.5ml each |
| High concentration standard 1ppm | 1.5ml |
|
HRP-conjugate |
12ml |
| Antibody Solution | 4ml |
| Substrate A | 6ml |
| Substrate B | 6ml |
| Stop Solution | 6ml |
| Concentrated Sample Extraction (Solution 18X) | 40ml |
| Concentrated Wash Buffer (10×) | 40ml |
| IFU | 1 |
| Testing Report | 1 |
Sensitivity, accuracy and precision
Sensitivity: 0.2ppb
Limit of detection
Honey………………………………………………………………………………………………2ppb
Percent Recovery, %
Honey, milk…………………………………………………………………………………90±15%
Precision (CV %):
Intra-lab assay: CV%≤10%
APPLICATIONS
Scope of Application
Qualitative or quantitative analysis the residues of Streptomycinin honey.
