Pammvi is the authorised suppliers, agents, dealers and wholesalers for Aflatoxin M1 ELISA test kit.
Aflatoxins belong to the class of mycotoxins. Chemically they are defined as difuranocyclopentanocumarines or difuranopentanolidocumarines, i.e. aflatoxins contain a dihydrofuran or a tetrahydrofuran ring, to which a substituted cumarin system is condensed. Out of about 20 known aflatoxins, the moulds Aspergillus flavus and A. parasiticus produce exclusively aflatoxin B1, B2, G1and G2, and all the other aflatoxins are derivates of these four. The derivates are developed either by metabolism in humans, animals and microorganisms or by environmental reactions.
Aflatoxin M1 was the first metabolite of Aflatoxin B1, which could unequivocally be detected by Allcroft and Carnaghan in the milk of cows in 1963. Out of this reason this first derivative was called Aflatoxin M1 (= milk). As further investigations showed, also other mammalians excrete Aflatoxin M1 in milk, feces and urine. Contaminations of milk and milk products can be hazardous for human beings, because M1 is similar to Aflatoxin B1 regarding its hepatotoxicity. M1 is only less carcinogenic.
In order to protect people against aflatoxin-induced diseases, there is a need for the qualitative and quantitative control of endangered foodstuff, besides appropriate hygienic precautions, which avoid the formation of aflatoxins. TheAflatoxin M1 ELISA is a quick, economical and sensitive method to detect aflatoxin M1 in milk and milk products. After an appropriate sample preparation, 40 samples can be tested in duplicate within 140 minutes.
In addition to the Aflatoxin M1 ELISA (DEAM1E01) and Aflatoxin B1 ELISA (DEAB1E01), Immunoaffinity Columns are available:
The Aflatoxin (total) Immunoaffinity Columns are used for the purification and enrichment of Aflatoxin (total) from different matrices. The resulting eluate can be quantitatively analyzed by various analytical techniques like the Aflatoxin B1 and M1 ELISA or HPLC.
Aflatoxin M1 ELISA
|Kit size||: 96 wells|
|Sample material||: milk, milk products|
|Sample preparation||: extraction, dilution, centrifugation|
|Sample volume||: 100 µl|
|Standard range||: 100 – 10.000 pg/ml|
|Incubation||: 1 h, 1 h (RT/shaker), 20 min (RT/dark)|
|Measuring system||: TMB 450 nm|
|Sensitivity||: < 10 pg/ml|