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coconut elisa

Coconut Allergen Elisa Test


Pammvi is the authorised suppliers, agents, dealers and wholesalers for Coconut Allergy ELISA Test.

The Cocos palm (Cocos nucifera) belongs to the family of Areaceae. With about 3.3% the fraction of proteins in coconut is relatively low compared to other nuts. Some of these proteins are known for being allergenic. Compared to other nuts these proteins are still slightly characterized. Coconut allergies are relatively seldom, but can be very distinct in particular cases.

For coconut-allergic persons hidden coconut allergens in food are a critical problem. Already very low amounts of coconut can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, coconut-allergic per­sons must strictly avoid the consumption of coconut containing food. Cross-contamination, most­ly in consequence of the production pro­cess, is often noticed. This explains why in many cases the existence of coconut residues in food cannot be excluded. For this reason sensitive detection systems for coconut residues in food­stuff are required.

The Coconut ELISA represents a highly sensitive detection system for coconut and is particularly capable of the quantification of residues in cookies, cereals, ice-cream, chocolate and sausage.

Principle of the Test

The Coconut quantitative test is based on the principle of the enzyme linked immu­nosorbent as­say. An antibody directed against coconut proteins is bound on the surface of a microtiter plate. Coconut con­taining sam­ples or standards are given into the wells of the microtiter plate. After 20 minutes incu­ba­tion at room temperature the wells are washed with di­luted washing solution to remove un­bound ma­te­rial. A peroxidase conjugated second antibody direc­ted against coconut proteins is given into the wells and after 20 minutes of incubation the plate is washed again. A substrate solution is added and in­cu­bated for 20 minutes, resulting in the develop­ment of a blue colour. The colour development is terminated by the addition of a stop solution, and the colour turns yel­low. The yellow colour is measured photo­me­trically at 450 nm. The concentra­tion of coconut is di­rectly proportional to the colour inten­sity of the test sample.

Enzyme Immunoassay for the quantitative determination of Coconut in food

Technology : ELISA
Kit size : 96 wells
Sample material : food
Sample preparation : extraction, dilution, centrifugation
Sample volume : 100 µl
Standard range : 2 – 30 ppm
Incubation : 20, 20 (RT), 20 min (RT/dark)
Measuring system : TMB at 450 nm
Sensitivity : 0.4 ppm
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