PROPERTIES
PRODUCT DESCRIPTION
FurancilinELISA Kitis an indirect competitive enzyme-labeled immunoassay. The Furancilinantigen is precoated on the wells. The precoated antigen compete the FURANCILIN antibody (antibody solution) with Furancilinin the sample, anti-Furancilinantibody binds to the FURANCILIN-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the FURANCILIN concentration of the samples is derived.
TECHNICAL SPECIFICATIONS
Product Name:Furancilin ELISA Kit
Specification: 96T
Storage Condition: 2-8°C
Restriction: For research use only!
Cross-reactivity
Furancilin Metabolites…………………………………………………100%
Furantoin Metabolites………………………………………………<0.1%
Furazolidone Metabolites……………………………………………<0.1%
Furaltadone Metabolites………………………………………………<0.1%
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 6 vials of standard | 1ml each |
| High Concentrated Standard (100ppb) | 1ml |
|
HRP-conjugate |
6ml |
| Antibody Solution | 9ml |
| Substrate A | 6ml |
| Substrate B | 6ml |
| Stop Solution | 6ml |
| Concentrated Wash Buffer (10×) | 40ml |
| Concentrated redissolving solution | 40ml |
| Derivatization Reagent (10×) | 1ml |
| IFU | 1 |
| Testing Report | 1 |
Sensitivity, accuracy and precision
Sensitivity: 0.05ppb
Limit of detection: 0.1ppb
Percent Recovery, %
Tissue……………………………………………75±10%
Honey……………………………………………70±10%
Milk………………………………………….……80±10%
Precision (CV %):
Intra-lab assay: CV%≤10%
READ INSTRUCTION MUNUAL COMPLETELY BEFORE USE!
1. Take out the reagents from the refrigerator and allow them to reach room temperature (20 to 25°C) prior to running the test. Shake up the reagents before use.
2. Place the appropriate number of test wells into a microwell holder. Be sure to re-seal unused wells in the zip-lock bag with desiccant stored under the temperature 2-8°C (Do not freeze).
3. Numbering: Number the microwells for the standards and samples separately. Running standards and samples in duplicate.
4. Using a pipette with disposable tips, add 20μl of standards and samples to the appropriate test wells. Be sure to use a clean pipet tip for each. Then dispense 50µl of HRP-enzyme and 80µl antibody solution into each test well in order.
5. Shake the plate gently to mix contents, cover the plate rack and incubate the test wells at 25°C keeping away from light for 30 minutes.
6. Open the cover on the plate and dump the contents of the wells into an appropriate waste container. Fill the wells to overflowing with wash buffer and dump (300µL each well). Repeat for a total of four to five washes (wait 10s between each wash). Following the last wash, tap the inverted wells onto absorbent paper to remove the last of the wash buffer.
7. Dispense 50µL Substrate A and 50µL Substrate B into each well in order. Shake the plate gently. Cover the plate rack and incubate the wells at 25°C for 15 minutes.
8. Dispense 50 µL of stop solution into each test well. Shake the plate rack gently to mix.
9. Read and record the absorbance of the wells at 450nm using a plate reader (dual wavelength 450/630nm detecting is advised) within 5 minutes.
APPLICATIONS
Scope of Application
Qualitative or quantitative analysis the residues of Furancilinin tissues, honey, milk, etc.
