PROPERTIES
PRODUCT DESCRIPTION
Product Name: Melamine (MEL) ELISA Kit
Specification: 96T
Storage Condition: 2-8°C
Restriction: For research use only!
TECHNICAL SPECIFICATIONS
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 6 vials of standard | 1ml each |
| High concentration standard 1ppm | 1ml |
|
HRP-conjugate |
6ml |
| Antibody Solution | 6ml |
| Substrate A | 6ml |
| Substrate B | 6ml |
| Stop Solution | 6ml |
| Concentrated Sample redissolving solution 16X | 12ml |
| Concentrated Wash Buffer (10×) | 40ml X2 |
| IFU | 1 |
| Testing Report | 1 |
Sensitivity, accuracy and precision
Sensitivity: 1ppb
Limit of detection
Milk powder……………………………………………………………………………………50ppb
Yoghourt….……………………………………………………………………………………10ppb
Fresh Milk………………………………………………………………………………………50ppb
Feed….…………………………………………………………………………………………50ppb
Egg….………………………………………………………………………………………….10ppb
Percent Recovery, %
Milk powder…………………………………………………………………………………100±20%
Yoghourt….…………………………………………………………………………………100±20%
Fresh Milk……………………………………………………………………………………100±20%
Feed….………………………………………………………………………………………100±20%
Egg….………………………………………………………………………………………..100±20%
Precision (CV %):
Intra-lab assay: CV%≤10%
Melamine ELISA Kitis an indirect competitive enzyme-labeled immunoassay. The tetracycline antigen is precoated on the wells. The precoated antigen compete the tetracycline antibody (antibody solution) with tetracycline in the sample, anti- tetracycline antibody binds to the tetracycline-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the tetracycline concentration of the samples is derived.
Scope of Application
Qualitative or quantitative analysis the residues of Melaminein milk, milk powder, dairy, egg and feed, etc.
Cross-reactivity
Melamine…………………………………………………100%
