PROPERTIES
PRODUCT DESCRIPTION
Product Name: Tetracyclines (TCs) ELISA Kit
Specification: 96T
Storage Condition: 2-8°C
Restriction: For resaerch use only!
Summary
Tetracyclines are antibiotics which inhibit the bacterial growth by stopping protein synthesis. They have been widely used for the past forty years as therapeutic agent in human and veterinary medicine but also as growth promoter in animal husbandry. But the abuse of antibiotics will lead to the residues in the livestock and indirectly affect the health of human being.
HPLC method is now widely used in the detection of tetracyclines residues, but its high cost and complex operation make it unacceptable.
The Tetracyclines (TCs) ELISA Kit produced by Mganech significantly reduces both the testing cost and the operating difficulties.
Use Principles
Tetracyclines (TCs) ELISA Kitis an indirect competitive enzyme-labeled immunoassay. The tetracycline antigen is precoated on the wells. The precoated antigen compete the tetracycline antibody (antibody solution) with tetracycline in the sample, anti- tetracycline antibody binds to the tetracycline-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the tetracycline concentration of the samples is derived.
TECHNICAL SPECIFICATIONS
Cross-reactivity
Tetracycline…………………………………………………100%
Doxycycline…………………………………………………170%
Aureomycin…………………………………………………230%
Oxytetracycline……………………………………………250%
Provided Materials and Reagent
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 6 vials of standard | 1ml each |
|
HRP-conjugate |
6ml |
| Antibody Solution | 6ml |
| Substrate A | 6ml |
| Substrate B | 6ml |
| Stop Solution | 6ml |
| Concentrated Sample Extraction Solution | 12ml |
| Concentrated Wash Buffer (10×) | 40ml |
| Concentrated redissolving solution | 40ml |
| IFU | 1 |
| Testing Report | 1 |
Sensitivity, accuracy and precision
Sensitivity: 0.05ppb
Limit of detection
Honey………………………………………………………………………………………………1ppb
Milk…………….……………………………………………………………………………………1ppb
Tissues (including aquatic products), egg………………………………………3ppb
Water Sample…………………………………………………………………………………1ppb
Percent Recovery, %
Honey, milk…………………………………………………………………………………90±10%
Tissues (including aquatic products), egg………………………………….90±15%
Water Sample………………………………………………………………………………80±10%
Precision (CV %):
Intra-lab assay: CV%≤10%
APPLICATIONS
Scope of Application
Qualitative or quantitative analysis the residues of tetracycline, oxytetracycline, aureomycin, doxycycline in honey, milk, tissues (including aquatic products), etc.
