Tetracyclines Elisa Test Kit

Overview:

Tetracyclines are antibiotics which inhibit the bacterial growth by stopping protein synthesis. They have been widely used for the past forty years as therapeutic agent in human and veterinary medicine but also as growth promoter in animal husbandry. But the abuse of antibiotics will lead to the residues in the livestock and indirectly affect the health of human being.

PROPERTIES

Tetracyclines are antibiotics which inhibit the bacterial growth by stopping protein synthesis. They have been widely used for the past forty years as therapeutic agent in human and veterinary medicine but also as growth promoter in animal husbandry. But the abuse of antibiotics will lead to the residues in the livestock and indirectly affect the health of human being.

PRODUCT DESCRIPTION

Product Name: Tetracyclines (TCs) ELISA Kit

Specification: 96T

Storage Condition: 2-8°C

Restriction: For resaerch use only!

Summary

Tetracyclines are antibiotics which inhibit the bacterial growth by stopping protein synthesis. They have been widely used for the past forty years as therapeutic agent in human and veterinary medicine but also as growth promoter in animal husbandry. But the abuse of antibiotics will lead to the residues in the livestock and indirectly affect the health of human being.

HPLC method is now widely used in the detection of tetracyclines residues, but its high cost and complex operation make it unacceptable.

The Tetracyclines (TCs) ELISA Kit produced by Mganech significantly reduces both the testing cost and the operating difficulties.

Use Principles

Tetracyclines (TCs) ELISA Kitis an indirect competitive enzyme-labeled immunoassay. The tetracycline antigen is precoated on the wells. The precoated antigen compete the tetracycline antibody (antibody solution) with tetracycline in the sample, anti- tetracycline antibody binds to the tetracycline-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the tetracycline concentration of the samples is derived.

TECHNICAL SPECIFICATIONS

Cross-reactivity

Tetracycline…………………………………………………100%

Doxycycline…………………………………………………170%

Aureomycin…………………………………………………230%

Oxytetracycline……………………………………………250%

Provided Materials and Reagent

Component	96 wells
Antigen coated plate	12 test strips of 8 wells each
6 vials of standard	1ml each
HRP-conjugate	6ml
Antibody Solution	6ml
Substrate A	6ml
Substrate B	6ml
Stop Solution	6ml
Concentrated Sample Extraction Solution	12ml
Concentrated Wash Buffer (10×)	40ml
Concentrated redissolving solution	40ml
IFU	1
Testing Report	1

Sensitivity, accuracy and precision

Sensitivity: 0.05ppb

Limit of detection

Honey………………………………………………………………………………………………1ppb

Milk…………….……………………………………………………………………………………1ppb

Tissues (including aquatic products), egg………………………………………3ppb

Water Sample…………………………………………………………………………………1ppb

Percent Recovery, %

Honey, milk…………………………………………………………………………………90±10%

Tissues (including aquatic products), egg………………………………….90±15%

Water Sample………………………………………………………………………………80±10%

Precision (CV %):

Intra-lab assay: CV%≤10%

APPLICATIONS

Scope of Application

Qualitative or quantitative analysis the residues of tetracycline, oxytetracycline, aureomycin, doxycycline in honey, milk, tissues (including aquatic products), etc.

Tetracyclines Elisa Test Kit

Tetracyclines Elisa Test Kit

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